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competitive pcr :3 products

hana fakhoury hfakhour at fs1.ho.man.ac.uk
Thu Dec 7 14:51:39 EST 1995

I am doing competitive quantitative RT-PCR where target DNA and standard are 
amplified together. The standard is similar to target sequence except for a 
small deletion. When I amplify each on its own , I have no problem and a 
single product is observed , but when the two are amplified together, at 
concentration that allow both to be amplified, I see a product amplified from 
target, a product from standard, and a third band in between the two that I 
can not relate to anything.
Has anyone came across this problem before ?
If yes, could you please advice on how to solve this problem.


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