Northern hybridisation

Chi-Kuang Wen cwen at aux.btny.purdue.edu
Thu Dec 7 01:34:11 EST 1995


Mr. Wilson wrote:davyjaw at liverpool.ac.uk (Mr D.J.A. Wilson) 
wrote:
>Pleas can anyone supply me with any information about what is the best way to
>hybridise a random-prime labelled DNA probe in a northern blot. I am having 
>real problems but I think the RNA is OK, and the probe laballing is OK, so it
>must be the hybridisation step. Any advice would be a great help.
>Thanks
>


Dear Mr. Wilson:

I usually use Church Solution and it works perfectly.

Best wishes,

Chi-Kuang


ps. by using Church sol. I even use deionied water, not the
Mili-Q water, to prepare the washing sol, and can repeat
hybridization for many times without affecting the result.

-------------- next part --------------
From: davyjaw at liverpool.ac.uk (Mr D.J.A. Wilson)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Northern hybridisation
Date: Wed, 6 Dec 1995 16:09:45 GMT
Organization: The University of Liverpool
Message-ID: <DJ6A8A.BrF at liverpool.ac.uk>

Pleas can anyone supply me with any information about what is the best way to
hybridise a random-prime labelled DNA probe in a northern blot. I am having 
real problems but I think the RNA is OK, and the probe laballing is OK, so it
must be the hybridisation step. Any advice would be a great help.
Thanks



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