Acrylamide Gel Solubilization

Flip Hoedemaeker fhoedem at oci.utoronto.ca
Fri Dec 8 14:49:08 EST 1995


If solubilisation doesn't work, isn't it possible to extract your DNA?
If you for instance wash your piece of gel in 4 vols buffer O/N you can 
count the DNA that comes out, and simply correct for the 20% that stays 
behind.... You won't need harsh methods like adding 30% peroxide!

Flip



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