Need a two-hybrid vector

Rui Lu lur at duke.usask.ca
Fri Dec 8 13:11:46 EST 1995


Hi, netters,

	I am trying to set up a yeast two-hybrid system in our lab. 
However, the presumed binding site of our protein lies at the extreme
N terminus. So I am looking for a vector that would "ligate" the C 
terminus of our protein to the Gal4 binding domain, instead of the 
conventional Gal4BD-protein order. That is: the multipul cloning site 
is at the  N end of Gal4?

	Who has it ? Giveme giveme giveme......  :)

--
Ray Lu
e-mail: lur at duke.usask.ca
TEL: (306)966-7237
FAX: (306)966-7244



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