Need a two-hybrid vector
lur at duke.usask.ca
Fri Dec 8 13:11:46 EST 1995
I am trying to set up a yeast two-hybrid system in our lab.
However, the presumed binding site of our protein lies at the extreme
N terminus. So I am looking for a vector that would "ligate" the C
terminus of our protein to the Gal4 binding domain, instead of the
conventional Gal4BD-protein order. That is: the multipul cloning site
is at the N end of Gal4?
Who has it ? Giveme giveme giveme...... :)
e-mail: lur at duke.usask.ca
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