gene replacement

Hank Seifert h-seifert at
Fri Dec 8 12:59:06 EST 1995

In article <4a9i4u$fuu at>, jn10001 at says...
>dear netters,
>I have mutated a cloned gene from a large conjugative plasmid
>and now I intend to replace the mutated gene in its original
>position. There's no way I can do that by cut and paste cloning
>so I must do it by in vivo gene replacement. Any one has
>direct experience on that kind of experiment. Any suggestions
>will be appreciated,
I think you need to provide more information about this before advice can be 
given. What vector is your mutated version in? What is the iriginal/final host 
Hank Seifert <h-seifert at>
Department of Microbiology-Immunology
Northwestern University

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