poly(A) RNA isolation, question?

John W. Rooney rooney at mbcrr.harvard.edu
Fri Dec 8 19:11:48 EST 1995

I and others I know have had good luck using Qiagen's Oligotex poly-dT 
coated beads, with which you can isolate poly A+ RNA similar to doing an 
immunoppt.  It's fast and has yielded good cDNA for me.  Also, it's not 
as expensive as kits--you can buy just the beads and make all the other 
solutions yourself.

Good luck--John Rooney, UC Berkeley

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