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gene replacement

Jose Nieto (Path) jn10001 at mole.bio.cam.ac.uk
Fri Dec 8 09:29:50 EST 1995

dear netters,

I have mutated a cloned gene from a large conjugative plasmid
and now I intend to replace the mutated gene in its original
position. There's no way I can do that by cut and paste cloning
so I must do it by in vivo gene replacement. Any one has
direct experience on that kind of experiment. Any suggestions
will be appreciated,


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