APS lovers!

Dima Klenchin klenchin at macc.wisc.edu
Fri Dec 8 09:48:28 EST 1995


In article <4a85kp$t4s at kadri.ut.ee>, mspeek at tamm.eenet.ee (Mart Speek) wrote:
>Dima Klenchin (klenchin at macc.wisc.edu) wrote:
>: In article <4a2sga$oj8 at kadri.ut.ee>, mspeek at tamm.eenet.ee (Mart Speek) wrote:

>: And I just can't resist to add: POLYMERIZATION TIME 
>: should be 30-45 min!
>
>>>>>> Hey Dima <<<<<
>Why 30-45 min? Uups! It depends which %%% gel you're casting!
>I agree it is good for 20% PAAG, but for lower % gels I'd

			^^^^^^^^
            I was talking about 10-15%
>always recommend quicker polymerization, like for 4-5% gels,
>5-8 min only! 

And I disagree. I once (for purpose) compared results with different
amounts of APS/TEMED so that po-n was ~ 10 and 40 min at 7%. 
No huge difference but banding was visibly better for "slow" 
polymerization. 

- Dima




More information about the Methods mailing list