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Effect of Solvent on Base Pairing?

Jean-Manuel Henry ez041797 at bullwinkle.ucdavis.edu
Fri Dec 8 03:57:29 EST 1995


Hi, 
   Apparently tertramethylammonium chloride will swamp out the AT vs. GC 
melting difference: a 100mer AT sequence will have the same 
melting temperature as a 100mer GC sequence.  Does anyone know the 
mechanism responsible for this observed effect? Also, does anyone know if 
guanidinium thiocyanate would have the same or a similar effect on double 
stranded melts ? I have consulted methodology texts but have not found 
the reason for the similar melt temp of AT and GC pairs, any help would 
be greatly appreciated. 
 
Jean-Manuel Henry 
University of California at Davis
Department of Molecular and Cellular Biology    



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