so many claims for so many kits -- how can we tell what works?

David L. Haviland, Ph.D. haviland at KIDS.WUSTL.EDU
Sat Dec 9 00:57:30 EST 1995


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Jon:

The subject line says it all.  You often can't. One must be VERY selective
in chosing a kit.  I've spent 8 years in molecular biology and quite
frankly, the only kits that I have any faith in are the ones in which I
can identify every component - transcription kits, cDNA libs, etc. 
Without fail or hesitation, the kits that I've had THE most trouble with
are those that have some magic proprietary ingredient "X" that is the key
to the whole protocol.  "X" often fails for a variety of reasons, wrong
bug, wrong vector, phase of the moon not right, etc.  But since we're not
told everything in the kit (the identity of "X"), it it almost impossible to 
pin point where the protocol failed. 

I'll stop here before I really get going...
David

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