Kay A. Pleyte (kpleyte at post.its.mcw.edu) wrote:
: I need to extract RNA from rat aorta. The problem is that most of the
: tissue in aorta is connective tissue and only the thin lining of the vessel
: is made up of the smooth muscle that I want. I can't use a lot of aortas to
: start with because the rats I'm using a special strain and are expensive.
: I will probably have to isolate total RNA, just to get a reasonable
: amount to pellet, and use a carrier. Does anyone have experience getting
: RNA from aorta.
I have, and its not that bad. I use the Trizol reagent sold by BRL (and
others under different names). The key is that the tissue must be
homogenized in a Brinkman Polytron or similar device. Don't Dounce!
Hope this helps. Good luck.