In article <4aat3b$j5n at epx.cis.umn.edu>, shin at biosci.cbs.umn.edu (Shin
> It's a modified Hanahan procedure. More simple and reliable. Key
> feature is growing the cells at 18C. Kudos to the authors for checking
> all the parameters.
>> I don't have the authors names, sorry. I switched to this about 2 years
> ago and have not gone near electroporators. My all time high is 10^10.
> We routinely get 10^8.
Inoue, H. Nojima, H. and Okkayama H.: Gene, 96 (1990) 23-27
Works very well.
Zophonias O. Jonsson
Institut fur Veterinarbiochemie Tel: (41-1)-257-54-75
Universitat Zurich-Irchel Fax: (41-1)-362-05-01