IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Ultracompetent E.coli cells?

Zophonias O. Jonsson zjons at vetbio.unizh.ch
Sat Dec 9 08:25:23 EST 1995

In article <4aat3b$j5n at epx.cis.umn.edu>, shin at biosci.cbs.umn.edu (Shin
Enomoto) wrote:
> It's a modified Hanahan procedure.  More simple and reliable.  Key 
> feature is growing the cells at 18C.  Kudos to the authors for checking 
> all the parameters.
> I don't have the authors names, sorry.  I switched to this about 2 years 
> ago and have not gone near electroporators.  My all time high is 10^10.  
> We routinely get 10^8. 

Inoue, H. Nojima, H. and Okkayama H.: Gene, 96 (1990) 23-27

Works very well.


Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-257-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-362-05-01
Winterthurerstrasse 190
CH-8057 Zurich

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net