the effect of tRNA on cDNA cloning ?

Seongwook Kwon swkwon at email.unc.edu
Fri Dec 8 13:45:15 EST 1995


Hi,

I am thinking of using tRNA to help out precipitating my cDNAs.  These 
cDNAs are to be used for a library construction.

	Does anyone know the effect of tRNA presence on 1) conversion of ss 
cDNA into ds cDNA (I will use Klenow), 2) ligation of insert DNA into 
lambda vector 3) packaging of vector-insert into lambda particles ?





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