In article <30C4D446.2F1B at umich.edu>, RNeubig at umich.edu says...
>I am interested in designing unique restriction sites into a
>general area of a plasmid without changing the expressed
>are no naturally occuring restriction sites. Two questions:
>>1) Is there software that tackles this kind of problem or are
>general approaches which have been taken to simplify this?
>>2) Is there a good place to get a list of restriction enzymes
>sorted by their recognition sequence (not by enzyme name)?
>would help me with this process.
In the appendix of New England Biolabs or Stratagene catalogue
you will find lists of RE sorted by recognition sequence.