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Designing in restriction sites

suihuang at usa1.com suihuang at usa1.com
Sat Dec 9 00:29:06 EST 1995

In article <30C4D446.2F1B at umich.edu>, RNeubig at umich.edu says...
>I am interested in designing unique restriction sites into a 
>general area of a plasmid without changing the expressed 
protein. There
>are no naturally occuring restriction sites. Two questions:
>1) Is there software that tackles this kind of problem or are 
>general approaches which have been taken to simplify this?
>2) Is there a good place to get a list of restriction enzymes
>sorted by their recognition sequence (not by enzyme name)? 
>would help me with this process.

In the appendix of New England Biolabs or Stratagene catalogue 
you will find lists of RE  sorted by recognition sequence.
Good luck,
Sui Huang 

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