IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

poly(A) RNA isolation, question?

Paul W. Diaz Pendragn at uclink2.berkeley.edu
Fri Dec 8 19:09:47 EST 1995

I and others I know have had good luck using Qiagen's Oligotex poly-dT 
coated beads, with which you can isolate poly A+ RNA similar to doing an 
immunoppt.  It's fast and has yielded good cDNA for me.  Also, it's not 
as expensive as kits--you can buy just the beads and make all the other 
solutions yourself.

Good luck--John Rooney, UC Berkeley

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net