In article <4a9lfi$6ic at mother.usf.edu>, acannons at com1.med.usf.edu says...
>>Does anyone have suggestions why our blots are suddenly getting a lot of
>background? We use random-prime labeled DNA with no purification of the
>probe. The background is speckled-like and is very pronounced around the
>edge of the blot.
>>Any ideas? Thanks
what is your blotting solution ? I solved the same problem by changing
from 10x SSC to 0.4M NaOH. It works even with non-charged nylon membranes.
I think that precipitated salt on the membrane led to the spotty
Or try a membrane with a lower binnding capacity, such as nitrocelluloses
Hope I could help you,