maieru at uni-muenster.de
Sat Dec 9 07:00:18 EST 1995
In article <4a9lfi$6ic at mother.usf.edu>, acannons at com1.med.usf.edu says...
>Does anyone have suggestions why our blots are suddenly getting a lot of
>background? We use random-prime labeled DNA with no purification of the
>probe. The background is speckled-like and is very pronounced around the
>edge of the blot.
>Any ideas? Thanks
what is your blotting solution ? I solved the same problem by changing
from 10x SSC to 0.4M NaOH. It works even with non-charged nylon membranes.
I think that precipitated salt on the membrane led to the spotty
Or try a membrane with a lower binnding capacity, such as nitrocelluloses
Hope I could help you,
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