Can you transfect RNA into cells?

Peter Wang plw at mrc-lmb.cam.ac.uk
Sat Dec 9 12:31:12 EST 1995


I was wondering about whether you can transfect RNA (produced in vitro 
by T7) into eucaryotic cells by any of the methods used for DNA 
(electroporation, lipofection, calcium phosphate, etc).
If so, will the RNA be expressed (assuming it has appropriate 5'mG cap)?
I know people have microinjected RNA into oocytes and had it expressed, 
but I was thinking of garden-variety tissue-culture cells.
Thanks for any insights!

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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402100  (international calls +44-1223-402100)
    (01223) 402104  (direct number)
Fax (01223) 402140
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