Can you transfect RNA into cells?
malexeyev at biost1.thi.tmc.edu
Sat Dec 9 18:08:27 EST 1995
In article <4ach50$s74 at lyra.csx.cam.ac.uk>, Peter Wang
<plw at mrc-lmb.cam.ac.uk> wrote:
> I was wondering about whether you can transfect RNA (produced in vitro
> by T7) into eucaryotic cells by any of the methods used for DNA
> (electroporation, lipofection, calcium phosphate, etc).
> If so, will the RNA be expressed (assuming it has appropriate 5'mG cap)?
> I know people have microinjected RNA into oocytes and had it expressed,
> but I was thinking of garden-variety tissue-culture cells.
> Thanks for any insights!
Try "Cationic liposome-mediated RNA transfection" by Dwarki et al. in
Meth. Enzymol. 1993 v.217:644-54
Also, to my knowlege RNA electroporation is widely used in plant systems.
I am not sure that cap is absolutely necessary, though.
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