David L. Haviland, Ph.D. (HAVILAND at KIDS.WUSTL.EDU) wrote:
: I contacted Sratagene on that very point over a year ago. Their reply was
: that thier protocol for peparing the ultracomps was nothing more than a
: modified form of the Hanahan protocol. However, these modifications were
: proprietary, of course, and would not be revealed - otherwise they'd loose
: thier market share! With standard CaCl2, I've managed only as high as 10^4
: I'd love to find a chemical protocol that would get me 10^8 on a regular
: basis. I should note that 10^8 is all I've ever managed to get with
: electroportation as well.
check out Gene96: 23
It's a modified Hanahan procedure. More simple and reliable. Key
feature is growing the cells at 18C. Kudos to the authors for checking
all the parameters.
I don't have the authors names, sorry. I switched to this about 2 years
ago and have not gone near electroporators. My all time high is 10^10.
We routinely get 10^8.