IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Ligase efficiency in different buffers ?

Darren Boehning boehnin1 at jeflin.tju.edu
Fri Dec 8 17:12:36 EST 1995

In article <stefanba-0712951947530001 at mun1.mun.uu.se>,
stefanba at bio.embnet.se (Stefan Bäckström) wrote:

> Hello.
> Does anyone know of a review on how well T4 DNA ligase works in different
> buffers ?
> Specially restriction buffers ?
> Thanks,
> Stefan Bäckström

We have found that NEB T4 works extremely well in NEB restriction buffer 4
supplemented with 1mM ATP (final concentration).  This is actually
suggested by the NEB supplemental materials that are sent with the ligase.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net