In article <stefanba-0712951947530001 at mun1.mun.uu.se>,
stefanba at bio.embnet.se (Stefan Bäckström) wrote:
>> Does anyone know of a review on how well T4 DNA ligase works in different
> buffers ?
> Specially restriction buffers ?
>> Stefan Bäckström
We have found that NEB T4 works extremely well in NEB restriction buffer 4
supplemented with 1mM ATP (final concentration). This is actually
suggested by the NEB supplemental materials that are sent with the ligase.