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Ligase efficiency in different buffers ?

Darren Boehning boehnin1 at jeflin.tju.edu
Fri Dec 8 17:12:36 EST 1995


In article <stefanba-0712951947530001 at mun1.mun.uu.se>,
stefanba at bio.embnet.se (Stefan Bäckström) wrote:

> Hello.
> 
> Does anyone know of a review on how well T4 DNA ligase works in different
> buffers ?
> Specially restriction buffers ?
> 
> Thanks,
> 
> Stefan Bäckström

We have found that NEB T4 works extremely well in NEB restriction buffer 4
supplemented with 1mM ATP (final concentration).  This is actually
suggested by the NEB supplemental materials that are sent with the ligase.



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