Ultracompetent E.coli cells?
horowicz at icrf.icnet.uk
Sat Dec 9 17:45:36 EST 1995
In article <4aat3b$j5n at epx.cis.umn.edu>, shin at biosci.cbs.umn.edu (Shin
> check out Gene96: 23
> It's a modified Hanahan procedure. More simple and reliable. Key
> feature is growing the cells at 18C. Kudos to the authors for checking
> all the parameters.
> I don't have the authors names, sorry. I switched to this about 2 years
> ago and have not gone near electroporators. My all time high is 10^10.
> We routinely get 10^8.
The authors are Inoue et al (1990). Agreed. We use it routinely for all
bacterial strains, except we grow at 25degC (doesn't require a cooled
incubator, and they grow faster). We usually get about 10^8.
ICRF Developmental Biology Unit, Oxford
d_horowicz at icrf.icnet.uk
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