Ultracompetent E.coli cells?

David Ish-Horowicz horowicz at icrf.icnet.uk
Sat Dec 9 17:45:36 EST 1995


In article <4aat3b$j5n at epx.cis.umn.edu>, shin at biosci.cbs.umn.edu (Shin
Enomoto) wrote:

> 
> check out Gene96: 23
> 
> It's a modified Hanahan procedure.  More simple and reliable.  Key 
> feature is growing the cells at 18C.  Kudos to the authors for checking 
> all the parameters.
> 
> I don't have the authors names, sorry.  I switched to this about 2 years 
> ago and have not gone near electroporators.  My all time high is 10^10.  
> We routinely get 10^8. 
 
The authors are Inoue et al (1990). Agreed. We use it routinely for all
bacterial strains, except we grow at 25degC (doesn't require a cooled
incubator, and they grow faster). We usually get about 10^8.

David Ish-Horowicz

David Ish-Horowicz
ICRF Developmental Biology Unit, Oxford
d_horowicz at icrf.icnet.uk



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