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Frozen competent yeast cell---Help

Seok-Won Yoon wonever at BORA.DACOM.CO.KR
Mon Dec 11 02:03:50 EST 1995

Hi netters.

Recently I'm using Pichia pastoris for protein expression.

What a drawback to use this yeast cell is that it's known to
impossible to make a frozen competent cell for storage.

Is there anyone know the special recipe to make a competent yeast
can store for a while?

I'd love to find a certain protocol on this matter.

Thank you in advance.

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