Tol Plasmids

Edgar Valencia edgar at cifn.unam.mx
Mon Dec 11 21:20:08 EST 1995


Hi A good technique for having a plasmid profile when plasmids are
big is as somebody else said the Eckhardt technique
Plasmid (1978)1:584-588 which is done using a vertical electropho
resis in 0.7% agarose gels
There is also a modification of this technique wich allows to 
run a horizontal gel Wheatcroft et al (1990)Mol. Plant Microbe 
Interactions 3:9-17.
Both methods have his advantages and disadvantages I prefer the
original protocol, its faster, the problem is to manage the 
gel before the run
the horizontal modification takes more time, but the bands are
better
Its up to you
I hope this helps


                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                                  MEXICO
                        e-mail edgar at cifn.unam.mx





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