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Tol Plasmids

Edgar Valencia edgar at cifn.unam.mx
Mon Dec 11 21:20:08 EST 1995

Hi A good technique for having a plasmid profile when plasmids are
big is as somebody else said the Eckhardt technique
Plasmid (1978)1:584-588 which is done using a vertical electropho
resis in 0.7% agarose gels
There is also a modification of this technique wich allows to 
run a horizontal gel Wheatcroft et al (1990)Mol. Plant Microbe 
Interactions 3:9-17.
Both methods have his advantages and disadvantages I prefer the
original protocol, its faster, the problem is to manage the 
gel before the run
the horizontal modification takes more time, but the bands are
Its up to you
I hope this helps

                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                        e-mail edgar at cifn.unam.mx

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