mRNA extraction from aorta?

J. Pat Martinez martinez at puccini.CRL.umn.edu
Mon Dec 11 11:11:49 EST 1995


The recent issue of Biotechniques has an article about extracting RNA from
Polysaccharide- and proteoglycan-rich sources.   The authors used rat
liver and aorta, among other things, in their experiments. 

Pat M.


 In article <4a9qco$1ek at news.duke.edu>, mmorales at acpub.duke.edu (Michael
Morales) wrote:

> Kay A. Pleyte (kpleyte at post.its.mcw.edu) wrote:
> :      I need to extract RNA from rat aorta.  The problem is that most of the
> : tissue in aorta is connective tissue and only the thin lining of the vessel
> : is made up of the smooth muscle that I want.  I can't use a lot of aortas to
> : start with because the rats I'm using a special strain and are expensive.
> 
> :      I will probably have to isolate total RNA, just to get a reasonable
> : amount to pellet, and use a carrier.  Does anyone have experience getting
> : RNA from aorta.
> 
> I have, and its not that bad.  I use the Trizol reagent sold by BRL (and 
> others under different names).  The key is that the tissue must be 
> homogenized in a Brinkman Polytron or similar device.  Don't Dounce!
> 
> Hope this helps.  Good luck.
> 
> Mike Morales



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