mRNA extraction from aorta?
J. Pat Martinez
martinez at puccini.CRL.umn.edu
Mon Dec 11 11:11:49 EST 1995
The recent issue of Biotechniques has an article about extracting RNA from
Polysaccharide- and proteoglycan-rich sources. The authors used rat
liver and aorta, among other things, in their experiments.
In article <4a9qco$1ek at news.duke.edu>, mmorales at acpub.duke.edu (Michael
> Kay A. Pleyte (kpleyte at post.its.mcw.edu) wrote:
> : I need to extract RNA from rat aorta. The problem is that most of the
> : tissue in aorta is connective tissue and only the thin lining of the vessel
> : is made up of the smooth muscle that I want. I can't use a lot of aortas to
> : start with because the rats I'm using a special strain and are expensive.
> : I will probably have to isolate total RNA, just to get a reasonable
> : amount to pellet, and use a carrier. Does anyone have experience getting
> : RNA from aorta.
> I have, and its not that bad. I use the Trizol reagent sold by BRL (and
> others under different names). The key is that the tissue must be
> homogenized in a Brinkman Polytron or similar device. Don't Dounce!
> Hope this helps. Good luck.
> Mike Morales
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