stripping Northern blots
sibley at genetics.washington.edu
Mon Dec 11 11:19:22 EST 1995
pmsmith at uniwa.uwa.edu.au (Penny Smith) wrote:
>I am using Hybond-N+ for Northern blots and have used the Amersham
>protocol for stripping them (ie pour on boiling 0.5% SDS and allow to cool
>to room temp.). This strips the blots but also seems to strip off the RNA
>as well. Does anyone have any suggestions.
>University of Western Australia
>pmsmith at uniwa.uwa.edu.au
We use Hybond-N for Northern blots and strip using 0.1X SSC and 0.1% SDS (boiling,
poured over blots and put on shaker to cool). How do you fix the RNA on the blot
prior to probing? We crosslink with UV and bake for 30 min. at 80C.
Genetics, University of Washington
torih at genetics.washington.edu
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