stripping Northern blots

Tori Brophy sibley at genetics.washington.edu
Mon Dec 11 11:19:22 EST 1995


pmsmith at uniwa.uwa.edu.au (Penny Smith) wrote:
>I am using Hybond-N+ for Northern blots and have used the Amersham
>protocol for stripping them (ie pour on boiling 0.5% SDS and allow to cool
>to room temp.).  This strips the blots but also seems to strip off the RNA
>as well.  Does anyone have any suggestions.
>
>Penny Smith
>Botany Dept.
>University of Western Australia
>pmsmith at uniwa.uwa.edu.au


We use Hybond-N for Northern blots and strip using 0.1X SSC and 0.1% SDS (boiling, 
poured over blots and put on shaker to cool).  How do you fix the RNA on the blot 
prior to probing?  We crosslink with UV and bake for 30 min. at 80C.  

Tori Brophy
Genetics, University of Washington
torih at genetics.washington.edu





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