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transfection of macrophages

brett brett at BORCIM.WUSTL.EDU
Mon Dec 11 23:47:02 EST 1995


>I would also like to know about such methods.   I am trying to transfect 
>J774 cells, and have had no luck with DEAE Dextran or lipofectamine.  Any 
>pointers would be appreciated.

I have used the BES-buffered CaPO4 technique to transfect J774, RAW264.7, and
P388.D1 cells stably, but haven't looked for transient expression. Will this
help?


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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