Subcloning vector for 13.5kb genomic fragment

Steven Goldberg goldberg at bms.com
Mon Dec 11 08:43:41 EST 1995


Strain DH10B from BRL was supposedly designed to accept and stably 
maintain large cloned fragments.  I have used it to transform plasmids 
such as pBluescript and pUC with inserts as large as 40 kb, so you might 
want to try it.

Steve





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