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stripping Northern blots

Penny Smith pmsmith at uniwa.uwa.edu.au
Mon Dec 11 05:21:15 EST 1995

I am using Hybond-N+ for Northern blots and have used the Amersham
protocol for stripping them (ie pour on boiling 0.5% SDS and allow to cool
to room temp.).  This strips the blots but also seems to strip off the RNA
as well.  Does anyone have any suggestions.

Penny Smith
Botany Dept.
University of Western Australia
pmsmith at uniwa.uwa.edu.au

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