I have a peptide with a C-terminal cysteine that I want to attach to
activated agarose beads. Can anybody suggest a good product for this
purpose? My peptide contains a tyrosine, so I don't want to use
anything that relies on iodoacetyl chemistry, and also I would prefer
the reaction to be irreversible. I then intend to use the immobolised
peptide for precipitation from cell lysates, so anything that has high
non-specific binding is also out of the question. I would be grateful
for any suggestions.