Peptide Immobilisation

Michael Daws Michael.Daws at ucmp.umu.se
Tue Dec 12 07:10:06 EST 1995


I have a peptide with a C-terminal cysteine that I want to attach to 
activated agarose beads.  Can anybody suggest a good product for this 
purpose?  My peptide contains a tyrosine, so I don't want to use 
anything that relies on iodoacetyl chemistry, and also I would prefer 
the reaction to be irreversible.  I then intend to use the immobolised 
peptide for precipitation from cell lysates, so anything that has high 
non-specific binding is also out of the question.  I would be grateful 
for any suggestions.





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