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His-tag fusion protein vector

Michael Daws Michael.Daws at ucmp.umu.se
Tue Dec 12 06:07:19 EST 1995


Hi Namjin

Novagen does a number of plasmid vectors that have a C-terminal His-Tag 
sequence that seem to work very well.  They are based on the pET 
expression system and give a highly inducible expression.  I am 
currently using their pET25b expression vector which contains the 
C-terminal His Tag sequence and directs expression to the periplasm and 
it works very well.

Mike.





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