mol.weight on SDS PAGE
Koen De Smet
kds30
Tue Dec 12 04:27:41 EST 1995
SDS polyacrylamide gels are often used for determination of
the molecular weight of a protein, although several proteins
are known that produce an over- or underestimate of their true
molecular weight on a SDS-PAG.
We study a glycoprotein that gives such an abberant molecular
weight on SDS-PAG. We would like to mention this in a
manuscript we are preparing. Does anybody know of a paper that
we can refer to that discusses the accuracy and shortcomings
of this technique for molecular weight determination (of
glycoproteins)?
Thanks
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