difficult ligation

Sharon Lefebvre slefebvr at umabnet.ab.umd.edu
Tue Dec 12 10:34:18 EST 1995


I need to ligate three cDNA fragments into a vector.  The inserts are 
approximately 600 bp each and the vector is 3.5 kb.  Each fragment has a 
unique restriction site on its end.  I haven't been successful in 
ligating all of the pieces together at once, or even just three at a 
time, and I was wondering if anyone has had any luck in ligating together 
three or more fragments at once.

Sharon Lefebvre




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