In Article <30C4D446.2F1B at umich.edu>, Rick Neubig <RNeubig at umich.edu> wrote:
>I am interested in designing unique restriction sites into a particular
>general area of a plasmid without changing the expressed protein. There
>are no naturally occuring restriction sites. Two questions:
>>1) Is there software that tackles this kind of problem or are there
>general approaches which have been taken to simplify this?
>Thanks in advance,
>Rick Neubig RNeubig at umich.edu>Pharmacology
>University of Michigan Phone (313) 763-3650
>http://www.umich.edu/~rneubig FAX (313) 763-4450
In the GCG software package you can use the program MAP with the line
command /SILENT to give you sites that won't change the protein sequence.