His-tag fusion protein vector

Marc Visconti marcvis at resunix.ri.sickkids.on.ca
Tue Dec 12 09:45:04 EST 1995


I also use the pET expression system, the pET-30 vector which has 
His-tags at the N- and C-terminus.  So far we've found that the 
pET30 vector allows for the most flexibility in terms of expression.  
You will also be pleased with the yield...very high.  Try contacting 
Novogen directly at 1-800-207-0144 and ask for Barbara.  She's great 
at explaining everything for you.

--marc



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