Long PCR and RACE

Kevin Clancy, PhD. clancy at eri.uchsc.edu
Tue Dec 12 23:02:27 EST 1995


Dear All,

Has anyone attempted to use any of the long PCR kits in a RACE protocol.
I would like to amplify a 12 kb cDNA clone. I know about 2 kb of the 3' 
end but none of the 5' end. What sizes should I expect if I use the 
Boehringer Long PCR kit for instance?
In addition, can anyone recommend a protocol/reverse transcriptase for 
this?
Thanks for the help.
Yours sincerely,
Kevin Clancy






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