Has anyone attempted to use any of the long PCR kits in a RACE protocol.
I would like to amplify a 12 kb cDNA clone. I know about 2 kb of the 3'
end but none of the 5' end. What sizes should I expect if I use the
Boehringer Long PCR kit for instance?
In addition, can anyone recommend a protocol/reverse transcriptase for
Thanks for the help.