FYHOU at MACC.WISC.EDU (Fang-Yao Hou) wrote:
>Hi, everybody on the methods-and-reagents list:
>>While I am working on searching info about using immobalized oligo d(T) to
>isolate poly A+ RNA, I realize there are two ways of separating bound and
>unbound materials. One is to use centrifuge and the other is to use magnetic
>separation. I am wondering if somebody has tried to immobalize oligo d(T) on a
>stick, for example paper stick, that we can simply dip this stick into the
>solution containing poly A+ RNA and transfer the stick to a washing well several
>times and finally release the bound poly A+ RNA in water. The way we may
>eliminate the need of either a centrifuge or a magnetic stand. Just an idea to
>share and welcome further discussion concerning its feasibility.
>FYHOU at MACC.WISC.EDU
Yes, Amersham has this product and I used it many years ago. It's called Hybond-mAP. The problem is that you still need to spin the the
paper to give a maximum recovery of bound RNA. Thereafter, fine paper fiber will be spun.