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an idea to share about fast isolation of poly A+ RNA

CK Wen cwen at aux.btny.purdue.edu
Tue Dec 12 17:05:07 EST 1995


FYHOU at MACC.WISC.EDU (Fang-Yao Hou) wrote:
>Hi, everybody on the methods-and-reagents list:
> 
>While I am working on searching info about using immobalized oligo d(T) to
>isolate poly A+ RNA, I realize there are two ways of separating bound and
>unbound materials. One is to use centrifuge and the other is to use magnetic
>separation. I am wondering if somebody has tried to immobalize oligo d(T) on a
>stick, for example paper stick, that we can simply dip this stick into the
>solution containing poly A+ RNA and transfer the stick to a washing well several
>times and finally release the bound poly A+ RNA in water. The way we may
>eliminate the need of either a centrifuge or a magnetic stand. Just an idea to
>share and welcome further discussion concerning its feasibility.
> 
>Fang-Yao Hou
>FYHOU at MACC.WISC.EDU

Dear Hou:
  Yes, Amersham has this product and I used it many years ago.  It's called Hybond-mAP.  The problem is that you still need to spin the the
paper to give a maximum recovery of bound RNA.  Thereafter, fine paper fiber will be spun.

Chi-Kuang

























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