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guanidinium thiocyanate?

Jean-Manuel Henry ez041797 at dale.ucdavis.edu
Tue Dec 12 03:53:29 EST 1995

   Apparently tertramethylammonium chloride will swamp out the AT vs. GC
melting difference: a 100mer AT sequence will have the same
melting temperature as a 100mer GC sequence.  Does anyone know the
mechanism responsible for this observed effect? Also, does anyone know if
guanidinium thiocyanate would have the same or a similar effect on double
stranded melts ? I have consulted methodology texts but have not found
the reason for the similar melt temp of AT and GC pairs, any help would
be greatly appreciated.

Jean-Manuel Henry
University of California at Davis
Department of Molecular and Cellular Biology

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