Tori Brophy <sibley at genetics.washington.edu> wrote:
>pmsmith at uniwa.uwa.edu.au (Penny Smith) wrote:
>>I am using Hybond-N+ for Northern blots and have used the Amersham
>>protocol for stripping them (ie pour on boiling 0.5% SDS and allow to cool
>>to room temp.). This strips the blots but also seems to strip off the RNA
>>as well. Does anyone have any suggestions.
>>University of Western Australia
>>pmsmith at uniwa.uwa.edu.au
We have used hot (bring to bubbly in microwave, then remove) 1% glyerol
plus 0.1% SDS. Just leave it there for 2 min, then wash the blot as
usual (1X SSC, 0.1% or whatever you use). Check to see that the signal
is truly removed by exposing O/N. This method is quite gentle and very
effective. It can even remove some dots and spots.