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sequencing with old 35S-dATP

Mr. I.S. Viney iviney at hgmp.mrc.ac.uk
Wed Dec 13 05:38:28 EST 1995



On 12 Dec 1995, Szatmari George wrote:

> Hi netters,
> 
> We recently did some sequencing reactions with some old 35S-dATP
> (the stuff was dated the 1st of May 95), which, much to my surprise,
> yielded some clean & readable sequence after a 5 day exposure to
> film (we used 2X the amount of label in the sequenase reactions).
> 
> Has anyone out there had similar results with old label?

Yes

Ian Viney
Imperial College




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