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sequencing with old 35S-dATP

CK Ng CNG at irp30.nidr.nih.gov
Wed Dec 13 15:02:59 EST 1995

szat at ERE.UMontreal.CA (Szatmari George) wrote:

>Hi netters,

>We recently did some sequencing reactions with some old 35S-dATP
>(the stuff was dated the 1st of May 95), which, much to my surprise,
>yielded some clean & readable sequence after a 5 day exposure to
>film (we used 2X the amount of label in the sequenase reactions).

>Has anyone out there had similar results with old label?

>BTW, our label was from Amersham


Yes. It worked pretty well. The same could be said for 33P-dATP.


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