Length of spacer in His-tag fusion vector
chung006 at mc.duke.edu
Wed Dec 13 14:10:58 EST 1995
I would like to thank you who have responded to my question about mammalian expression
vectors with C-terminal His-tag. In addition to Invitrogen vectors which with N-terminal
His-tag, most of responses referred Qiagen and Novagen for C-terminal His-tag fusion
protein vectors. They do have C-terminal vectors, but not for mammalian expression.
I wonder I should construct C-terminal His-tag vectors by myself. At this moment, I would
ask you all again about proper length of amino acid spacer between C-terminus of my
protein and hexahistidine residues. Someone told me that 5-10 aa would be good for
N-terminal fusion, but I really don't know if this is also good for C-terminal fusion. Thank
you for suggestions in advance.
Duke University Medical Center
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