In article <4ajfu9$ggr at mark.ucdavis.edu>, ez041797 at dale.ucdavis.edu
(Jean-Manuel Henry) wrote:
> Apparently tertramethylammonium chloride will swamp out the AT vs. GC
> melting difference: a 100mer AT sequence will have the same
> melting temperature as a 100mer GC sequence. Does anyone know the
> mechanism responsible for this observed effect? Also, does anyone know if
> guanidinium thiocyanate would have the same or a similar effect on double
> stranded melts ?
For the mechanism of TMACl action on nucleic acids and the conditions for
its use you may find more information in Wood W.I. et al. PNAS
82:1585-1588 (1985) and Jacobs K.A. et al. NAR 16:4637-4650 (1988) and
older refs therein ('69+ '73). I have used it myself-unsuccessfully
No idea whether GTC works the same.
Axel Schumacher, Ph.D. ! Phone:+49 9131 852002
Institute for Pharmacology ! FAX: +49 9131 206119
University of Erlangen !
D-91054 Erlangen Germany ! as at macpost.pharmakologie.uni-erlangen.de