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guanidinium thiocyanate?

Axel Schumacher as at macpost.pharmakologie.uni-erlangen.de
Wed Dec 13 13:07:43 EST 1995


In article <4ajfu9$ggr at mark.ucdavis.edu>, ez041797 at dale.ucdavis.edu
(Jean-Manuel Henry) wrote:

> Hi,
>    Apparently tertramethylammonium chloride will swamp out the AT vs. GC
> melting difference: a 100mer AT sequence will have the same
> melting temperature as a 100mer GC sequence.  Does anyone know the
> mechanism responsible for this observed effect? Also, does anyone know if
> guanidinium thiocyanate would have the same or a similar effect on double
> stranded melts ?

For the mechanism of TMACl action on nucleic acids and the conditions for
its use you may find more information in Wood W.I. et al. PNAS
82:1585-1588 (1985) and Jacobs K.A. et al. NAR 16:4637-4650 (1988) and
older refs therein ('69+ '73). I have used it myself-unsuccessfully
though...
No idea whether GTC works the same.

Axel

-- 
Axel Schumacher, Ph.D.     ! Phone:+49 9131 852002
Institute for Pharmacology ! FAX:  +49 9131 206119
University of Erlangen     !
Universitaetsstr.22        !
D-91054 Erlangen  Germany  ! as at macpost.pharmakologie.uni-erlangen.de



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