We have linearized-plasmids that we have used as templates for transcription
of ion channel genes. A year ago, we found that the plasmids gave very good
expression. Today, a year later, we are having difficulty getting good
quality RNA. The plasmids were kept at 4 C. Should they still be viable?
J. David Spafford,
Department of Biological Sciences, University of Alberta
jspaffor at gpu.srv.ualberta.ca