I want to amplify DNA using primers as short as posibly. Does anybody
know what is minimal length of primer for DNA polymerase? I wonder if a
restriction enzyme cleavge site seuqence could be chosen as a primer
assuming no interanl site. it is known that dimer can be formed under
certein PCR conditions if one or two base(s) of two primers matching at
3'-end. Does it mean Thermal DNA polymerase can use shorter primers
compared to normal DNA polymerase? Any suggestions are wellcome. Thanks.