stripping Northern blots
ksnowden at bio.tamu.edu
Fri Dec 15 11:50:25 EST 1995
I have used hybond-N+ to do quite a few northerns. If you use alkali
transfer you do not need to use UV to crosslink your RNA to the membrane.
Using alkali transfer, i have stripped (in boiling 0.1% SDS) northerns and
reprobed them up to 8 times with no problems. For alkali transfer of RNA,
i use 50 mM NaOH, and capillary blot for 3-4 hours. I usually get
complete transfer after this time. Longer transfer times are detrimental
to the blot however.
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