<4a7vmd$837 at news.cais.com>, rover at ns1.livnet.com (Rover) writes:
> We pour at the same concentration you do but we cool or LB agar to 65
> deg C and then wait until its cool enough to manipulate with our hands
> before adding the ampicillin. We still experience satellites on our
we cool to 55C before pouring. Add Amp (50 mg/ml stock stored at -20C. Use
anhydrous, made up in 1 M NaHCO3) and roll bottles to mix. Then pour. Nah
>> --Rover The Mad Molecular Biologist
You think _you're_ mad ? ;-)
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
"Winds light to variable"