Staining DGGE gels with syber green?

bmay ndxdbmay at rrzn.uni-hannover.de
Thu Dec 14 16:52:01 EST 1995


Mats Grahn <Mats.Grahn at zooekol.lu.se> wrote:
> Hi,
> I'm currently staining my DGGE gels (5% a-amide) with EtBr with
> quite good results, but I plan to test the less toxic(?) syber
> green instead. Has anyone tried it, and are you willing to send
> me a protocol for staining?

I'm using SYBR-Green for SSCP and it works quite well
using the manufacturers instruction, but I don't think
it is less mutagenic and I know it is more than 100 times
more expensive than EtBr. So if EtBr works, don't change.

Bernhard Mayr
Dept. Clin. Endocrinology
Medizinische Hochschule Hannover
Hannover, Germany



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