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ThioFusion Expression System

Pieterke Groot at rulsfb.leidenuniv.nl
Fri Dec 15 12:24:02 EST 1995

Who has experience with the ThioFusion Expression System for protein 
purification, which is sold by Invitrogen? Is the fusion protein mainly 
expressed in the soluble fraction? And is it possible to excrete the 
proteins by osmotic shock treatment? And what is the best way to get rid 
of the Thioredoxin in the protein mixture? Hopefully anyone can give me 
some good advice. Thank you,


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