Migration of protein in SDS PAGE gel

Tang Hsin Yao mcbthy at leonis.nus.sg
Fri Dec 15 20:05:55 EST 1995


Hi! I have a problem here and I hope somebody out there can help me. I
have a protein with a predicted molecular mass of 150 kD. However when I
ran a SDS PAGE gel, it migrates at the size of 200 kD. Is there any good
explaination for this? I have read somewhere that certain proteins rich
in certain amino acid residues will move slower than their predicted
size. Is this true? I hope someone will point me to the references regarding
this matter.
        Thanks a lot in advance.



Hsinyao Tang
mcbthy at leonis.nus.sg




More information about the Methods mailing list