Standard protocol of Sequencing reactions with unlabeled primer and labeled
dATP using Sequenase use a minimum of 5 microgram/reaction.
Has anyone tried using 2 micrograms or below ??
Will increasing the amount of primer (to say 50 pmoles/reaction) help the reaction
produce better banding.
If someone has an idiot proof protocol on these lines, can you please post them
in this newsgroup.