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DNA concentrations in Sequenase X'ns ??

grant at grant at
Fri Dec 15 16:21:00 EST 1995

Standard protocol of Sequencing reactions with unlabeled primer and labeled 
dATP using Sequenase use a minimum of 5 microgram/reaction.
Has anyone tried using 2 micrograms or below ??
Will increasing the amount of primer (to say 50 pmoles/reaction) help the reaction
produce better banding.
If someone has an idiot proof protocol on these lines, can you please post them
in this newsgroup.
Thank you 

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